2016年6月在丹麦举行的第21届欧洲血液年会（EHA）中，有不少研究机构的科学家再次证明了免疫细胞受体（TCR/BCR）高通量（HTS）测序在微小残留病（MRD）检测中，比流式细胞仪（Flow Cytometry）更敏感，可作为判定治疗效果的超早期指标。
以下为部分会议内容：
Abstract #S101: Improved Efficacy after Incorporating Autologous Stem Cell Transplant (ASCT) into KRD Treatment with Carfilzomib (CFZ), Lenalidomide (LEN), and Dexamethasone (DEX) in Newly Diagnosed Multiple Myeloma
Presenter: Dr. Andrzej Jakubowiak
Date/Time: June 10, 11:45-12; Hall A1
Conclusion
Recognizing limitations of cross-study comparisons, KRd+ASCT shows superior outcomes vs historical KRd w/o ASCT, supporting further evaluation in the randomized setting. Both KRd studies compare favorably to other NDMM studies.
Abstract #S496: High Throughput Sequencing as a Measure of Early Response to Therapy in Childhood ALL
Presenter: Dr. Ilan Kirsch
Date/Time: June 11, 16-16:15; Auditorium 2
Conclusion
This is the largest patient cohort studied to date for which mpFC, HTS, and outcome data are available. This work suggests that HTS is an accurate and standardized assay whose increased sensitivity compared to mpFC is relevant to determination of patient outcome.
Abstract #E854: Molecular Detection of ALL in the Peripheral Blood is More Sensitive than Flow Cytometric Analysis of the Bone Marrow in Patients with Treatment-Related Hypocellularity
Author: Takamatsu, et al.
Conclusion
The impact of B cell aplasia may be important in increasing the sensitivity of HTS in the detection of MRD. The ability to detect an ALL B cell clone in the blood through HTS has many clinical implications; blood can be sampled more often, is less of a burden to patients, and can provide earlier prediction of relapse.
Abstract #E1293: Comparison between 8-Color Multiparameter Flow Cytometry and Next-Generation Sequencing to Detect Minimal Residual Disease in Multiple Myeloma Patients who Underwent Auto-SCT
Author: Kirsch, et al.
Conclusion
In this comparison study of MRD assessment in autografts using Euroflow and NGS approaches, the NGS platform showed higher sensitivity and prognostic value than EuroFlow.
Abstract #E1148: High Throughput TCR Sequencing Provides Added Value in the Diagnosis of Cutaneous T-cell Lymphoma
Author: Kirsch, et al.
Conclusion
Multiplex PCR and high throughput sequencing of TCRG and TCRB loci in suspect skin lesions was able to impact and add value to the diagnosis and monitoring of patients with early stage cutaneous T-cell lymphoma.
欧洲血液协会是致力于促进血液学临床实践、研究和教育的非盈利组织，1992创立于比利时，从1998年开始，欧洲血液协会会议成为了一年一度 的盛会，大会涵盖了多个学科并邀请行业极富盛名的专家和学者做大会演讲，目前协会有来自世界100个国家超过3000名会员。届时将是血液学家、医师和教 授交流和互动的最佳时机。
2016年第21届欧洲血液年会于6月9-12日在丹麦(哥本哈根)召开。
二、主办单位：欧洲血液协会
三、组委会成员：
欧洲血液协会主席：Christine Chomienne
欧洲血液协会副主席：Tony Green
四、组织单位：联合国际医院协作中心
五、活动地点：丹麦-哥本哈根